3SOJ

Francisella tularensis pilin PilE

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0, 0.9
Spacegroup name	P 21 21 21
Unit cell lengths	33.887, 62.160, 82.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.400 - 1.000
R-factor	0.1608
Rwork	0.160
R-free	0.18130
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.366
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400
High resolution limit [Å]	1.000	1.000
Number of reflections	94896
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	38% PEG 2000 MME 5% saturated LiSO4 25mM Bis-Tris propane 20mM Xylitol 10mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	7	295	38% PEG 2000 MME 5% saturated LiSO4 25mM Bis-Tris propane 20mM Xylitol 10mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K