3SOG
Crystal structure of the BAR domain of human Amphiphysin, isoform 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9173 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 56.710, 56.710, 164.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.070 - 2.300 |
R-factor | 0.2403 |
Rwork | 0.239 |
R-free | 0.26580 |
Structure solution method | SIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.110 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.025 |
Number of reflections | 13758 |
Completeness [%] | 99.1 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 277.15 | 2.66% PEG 6000, 2.66% PEG 8000, 2.66% PEG 10,000, 0.02M Magnesium Acetate, 0.05M Potassium Chloride, 0.1M MES, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |