3SOG

Crystal structure of the BAR domain of human Amphiphysin, isoform 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9173
Spacegroup name	P 31 1 2
Unit cell lengths	56.710, 56.710, 164.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.070 - 2.300
R-factor	0.2403
Rwork	0.239
R-free	0.26580
Structure solution method	SIRAS
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.110
High resolution limit [Å]	2.300
Rmerge	0.025
Number of reflections	13758
Completeness [%]	99.1
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	277.15	2.66% PEG 6000, 2.66% PEG 8000, 2.66% PEG 10,000, 0.02M Magnesium Acetate, 0.05M Potassium Chloride, 0.1M MES, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K