3SOD

CHANGES IN CRYSTALLOGRAPHIC STRUCTURE AND THERMOSTABILITY OF A CU,ZN SUPEROXIDE DISMUTASE MUTANT RESULTING FROM THE REMOVAL OF BURIED CYSTEINE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	92.500, 89.400, 70.500
Unit cell angles	90.00, 95.70, 90.00

Refinement procedure

Resolution	8.000 * - 2.100
R-factor	0.19 *
Rwork	0.190
RMSD bond length	0.030
RMSD bond angle	4.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.099 *
Total number of observations	60573 *
Number of reflections	28705 *
Completeness [%]	82.0 *	68 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	49 (%(v/v))
2	1	1	potassium phosphate	30 (mM)
3	1	1	antibiotic-antimycotic	0.5 (%)
4	1	1	protein	10 (mg/ml)