3SNO

Crystal structure of a putative aminotransferase (NCgl2491) from Corynebacterium glutamicum ATCC 13032 at 1.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97949,0.97932
Spacegroup name	C 1 2 1
Unit cell lengths	139.899, 52.586, 37.350
Unit cell angles	90.00, 98.92, 90.00

Refinement procedure

Resolution	28.757 - 1.600
R-factor	0.232
Rwork	0.231
R-free	0.25290
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE (January 30, 2009)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.757		1.660
High resolution limit [Å]	1.600	3.440	1.600
Rmerge	0.027	0.018	0.456
Number of reflections	34491	6515	5736
<I/σ(I)>	12.53	31.3	1.7
Completeness [%]	92.4	93	79.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.64	277	10.0% 2-methyl-2,4-pentanediol, 0.1M citric acid pH 3.64, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K