3SNM

Crystal structure of a lectin from Canavalia maritima seeds complexed with Indole-3-Acetic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-10
Detector	MAR CCD 130 mm
Wavelength(s)	1.433
Spacegroup name	I 2 2 2
Unit cell lengths	67.190, 70.740, 97.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.360 - 2.150
R-factor	0.20714
Rwork	0.206
R-free	0.22539
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.026
RMSD bond angle	2.654
Data reduction software	iMOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.360
High resolution limit [Å]	2.150
Number of reflections	11903
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Buffer Tris 0.1 M pH 8.5, PEG 4000 30%, lithium sulphate 0.2M, VAPOR DIFFUSION, HANGING DROP, temperature 293K, temperature 293.0K