3SNH

Crystal structure of nucleotide-free human dynamin1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-20
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	C 2 2 21
Unit cell lengths	155.056, 201.993, 59.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.590 - 3.700
R-factor	0.2903
Rwork	0.288
R-free	0.33050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2AKA 2DYN and 3LJB
RMSD bond length	0.007
RMSD bond angle	0.982
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.800
High resolution limit [Å]	3.700	3.700
Number of reflections	10214
<I/σ(I)>	13.1	2.14
Completeness [%]	99.2	99.7
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	9% PEG400, 6% isopropanol, 100 mM HEPES/NaOH (pH 7.3), 10 mM MgCl2, 10 mM KCl. , VAPOR DIFFUSION, SITTING DROP, temperature 277K