3SMZ
Human raver1 RRM1-3 domains (residues 39-320)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 104.020, 104.020, 50.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.990 |
| R-factor | 0.1877 |
| Rwork | 0.185 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h2u chain B |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.1.4) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.090 | 90.090 | 2.100 |
| High resolution limit [Å] | 1.990 | 6.310 | 1.990 |
| Rmerge | 0.034 | 0.014 | 0.503 |
| Number of reflections | 21054 | ||
| <I/σ(I)> | 29.4 | 94.8 | 2.1 |
| Completeness [%] | 98.5 | 99.9 | 90 |
| Redundancy | 6.7 | 6.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100 mM Tris-HCl (pH 8.5), 24% PEG 3350, 200 mM Lithium sulfate, Vapor diffusion, sitting drop, temperature 298K, VAPOR DIFFUSION, SITTING DROP |
