3SMA
A new N-acetyltransferase fold in the structure and mechanism of the phosphonate biosynthetic enzyme FrbF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 128.832, 35.692, 136.087 |
| Unit cell angles | 90.00, 117.60, 90.00 |
Refinement procedure
| Resolution | 45.340 - 2.000 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.340 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 74767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 291 | 0.2M magnesium chloride, 0.1 Tris, 20%-25% peg 4000, 20% glycerol, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
