3SLT
Pre-cleavage Structure of the Autotransporter EspP - N1023S Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.162, 122.372, 122.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.590 - 2.460 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.520 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.560 |
| High resolution limit [Å] | 2.460 | 5.320 | 2.470 |
| Rmerge | 0.079 | 0.036 | 0.590 |
| Number of reflections | 17404 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 98.9 | 98.8 | 97.9 |
| Redundancy | 5.4 | 5.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 20% w/v PEG8000, 20% v/v glycerol, sodium acetate, pH 7.5, HANGING DROP, temperature 294K |
