3SLR
Crystal structure of N-terminal part of the protein BF1531 from Bacteroides fragilis containing phosphatase domain complexed with Mg.
Replaces: 3NVBExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.741, 125.998, 41.622 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.870 - 1.712 |
| R-factor | 0.1878 |
| Rwork | 0.186 |
| R-free | 0.21320 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.977 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.870 |
| High resolution limit [Å] | 1.712 |
| Number of reflections | 42478 |
| Completeness [%] | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% PENTAERYTHRITOL PROPOXYLATE, 0.05M HEPES, 0.2M POTASSIUM CHLORIDE, 2mM MAGNESIUM CHLORIDE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
