3SLQ
Crystal structure of the 2'- Deoxyguanosine riboswitch bound to guanosine-5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.876, 48.390, 228.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.500 |
| R-factor | 0.2419 |
| Rwork | 0.237 |
| R-free | 0.28540 |
| Structure solution method | Molrep |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.376 |
| Number of reflections | 13677 | |
| <I/σ(I)> | 27.6 | 5 |
| Completeness [%] | 96.5 | 98.8 |
| Redundancy | 4.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | 0.05 M Na-succinate, ~3.0 M (NH4)2SO4, 0.5 mM spermine and 20 mM MgCl2., VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.1 |
