3SLO
Pre-cleavage Structure of the Autotransporter EspP - N1023D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.140, 121.664, 122.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.420 - 2.520 |
| R-factor | 0.2107 |
| Rwork | 0.208 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.820 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.610 |
| High resolution limit [Å] | 2.520 | 5.430 | 2.520 |
| Rmerge | 0.076 | 0.030 | 0.570 |
| Number of reflections | 16442 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 98.6 | 99.1 | 95.6 |
| Redundancy | 4.5 | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 18% w/v PEG8000, 20% v/v glycerol, 25 mM sodium acetate, pH 7.5, HANGING DROP, temperature 294K |
