3SLN
Structural characterization of a GII.4 2004 norovirus variant (TCH05) bound to H pentasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 242.220, 339.028, 124.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.850 - 2.841 |
| R-factor | 0.18899 |
| Rwork | 0.187 |
| R-free | 0.23116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sld |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.841 | 2.841 |
| Rmerge | 0.122 | |
| Number of reflections | 119306 | |
| <I/σ(I)> | 15.94 | |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295.15 | 0.8M Potassium sodium tartarate, 100mM TRIS, 0.5% w/v PEG monomethyl ether 5000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
