3SLE
Crystal Structure of the P107C-MauG/pre-Methylamine Dehydrogenase Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.851, 88.530, 107.698 |
| Unit cell angles | 116.21, 91.83, 99.35 |
Refinement procedure
| Resolution | 47.860 - 2.520 |
| R-factor | 0.17792 |
| Rwork | 0.174 |
| R-free | 0.24339 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3l4m |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.5.0109) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.062 | 0.241 |
| Number of reflections | 59309 | |
| <I/σ(I)> | 13.85 | 3.44 |
| Completeness [%] | 97.2 | 94.9 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 0.1M MES pH 6.4, 0.1M sodium acetate, 24-30 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
