3SL7

Crystal structure of CBS-pair protein, CBSX2 from Arabidopsis thaliana

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97888, 0.97928, 0.95000
Spacegroup name	P 65 2 2
Unit cell lengths	73.045, 73.045, 253.453
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.130 - 1.905
R-factor	0.2244
Rwork	0.223
R-free	0.24630
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.153
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: dev_589))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.905
Number of reflections	31938
Redundancy	25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1M Bis-Tris, 0.2M Calcium acetate, 22% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.1M Bis-Tris, 0.2M Calcium acetate, 22% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K