3SL2
ATP Forms a Stable Complex with the Essential Histidine Kinase WalK (YycG) Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | I 41 |
| Unit cell lengths | 59.040, 59.040, 99.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.900 - 1.610 |
| R-factor | 0.20865 |
| Rwork | 0.207 |
| R-free | 0.23412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c2a |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.592 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 1.660 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.023 | 0.302 |
| Number of reflections | 22011 | 2036 |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 287 | 10 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 1.5 mM NaN3, pH 7.5, VAPOR DIFFUSION, temperature 287K |
