3SKX
Crystal structure of the ATP binding domain of Archaeoglobus fulgidus COPB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97920, 0.9792, 0.9116, 0.9793 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 36.052, 62.682, 106.059 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 106.060 - 1.590 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23316 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.225 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.060 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.033 | 0.237 |
| Number of reflections | 31479 | |
| <I/σ(I)> | 25 | 4.9 |
| Completeness [%] | 95.3 | 93.8 |
| Redundancy | 5.63 | 4.61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M Sodium acetate, 0.1M TRIS.HCl pH 8.5, 30% PEG 4000, 0.01M Magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
