3SKI
Crystal structure of the 2'- Deoxyguanosine riboswitch bound to 2'-deoxyguanosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.080901 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.930, 47.795, 228.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.25616 |
| Rwork | 0.253 |
| R-free | 0.28134 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.079 | 0.278 |
| Number of reflections | 16645 | |
| Completeness [%] | 93.1 | 89.4 |
| Redundancy | 6.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.05 M Na-acetate or Na-succinate, pH 4.7-5.1, ~3.0 M ammonium sulfate, 0.5 mM spermine and 20 mM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
