3SK4
Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-14 |
| Detector | BRUKER APEX II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.213, 60.510, 38.626 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 31.120 - 1.850 |
| R-factor | 0.1766 |
| Rwork | 0.174 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.542 |
| Data reduction software | XPREP (2005/2 for Windows) |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.510 | 60.510 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.090 | 1.850 |
| Rmerge | 0.032 | 0.009 | 0.339 |
| Number of reflections | 12309 | 619 | 357 |
| <I/σ(I)> | 29 | ||
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 12.5 | 20.14 | 5.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
