3SJR
Crystal structure of conserved unkown function protein CV_1783 from Chromobacterium violaceum ATCC 12472
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915, 0.97929 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.366, 109.471, 151.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.940 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26740 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.206 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.120 | 0.834 |
| Number of reflections | 17091 | |
| <I/σ(I)> | 35 | 2.44 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.7 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 10mM tri-Sodium Citrate, 33%(w/v) PEG6000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
