3SIX

Crystal structure of NodZ alpha-1,6-fucosyltransferase soaked with GDP-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97242
Spacegroup name	P 65 2 2
Unit cell lengths	128.800, 128.800, 91.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.350
R-factor	0.2124
Rwork	0.210
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hhc
RMSD bond length	0.018
RMSD bond angle	1.491
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.091	0.045	0.656
Number of reflections	19190
<I/σ(I)>	20.5		3.3
Completeness [%]	99.9	98.8	100
Redundancy	10.6	10.8	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	350 mM potassium sodium tartrate 100 mM MES pH 6.5 50 mM MgCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 293K