3SIW
Crystal structure of NodZ alpha-1,6-fucosyltransferase co-crystallized with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 123.900, 123.900, 95.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.730 - 1.980 |
R-factor | 0.1737 |
Rwork | 0.172 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hhc |
RMSD bond length | 0.019 |
RMSD bond angle | 1.698 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.050 |
High resolution limit [Å] | 1.980 | 4.260 | 1.980 |
Rmerge | 0.119 | 0.068 | 0.706 |
Number of reflections | 30783 | ||
<I/σ(I)> | 16.6 | ||
Completeness [%] | 99.9 | 99.3 | 99.9 |
Redundancy | 9.8 | 9.1 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 400 mM KH2PO4 100 mM MES pH 6.5 5 mM MgCl2 2 mM GDP 1 mM Chitopentaose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |