3SIH
The X-ray crystal structure of poly(ADP-ribose) glycohydrolase (PARG) from Thermomonospora curvata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.245, 50.074, 105.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.156 - 1.500 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.006 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.803 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 37023 |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SITTING DROP, VAPOR DIFFUSION | 9 | 10% PEG6000, 0.1 M Bicine, pH 9.0, SITTING DROP, VAPOR DIFFUSION |
