3SIC

MOLECULAR RECOGNITION AT THE ACTIVE SITE OF SUBTILISIN BPN': CRYSTALLOGRAPHIC STUDIES USING GENETICALLY ENGINEERED PROTEINACEOUS INHIBITOR SSI (STREPTOMYCES SUBTILISIN INHIBITOR)

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	77.200, 185.900, 69.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.800
R-factor	0.178
RMSD bond length	0.016
RMSD bond angle	0.038 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.086 *
Total number of observations	62627 *
Number of reflections	19948 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	42.2 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)