3SIB

Crystal structure of URE3-binding protein, wild-type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97946
Spacegroup name	I 4 2 2
Unit cell lengths	99.699, 99.699, 107.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.160 - 1.900
R-factor	0.161
Rwork	0.159
R-free	0.19000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sia
RMSD bond length	0.017
RMSD bond angle	1.419
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.900	4.350	1.900
Rmerge	0.083	0.025	0.513
Number of reflections	21570
<I/σ(I)>	9
Completeness [%]	99.8	98.2	99.8
Redundancy	7.9	7.6	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	Internal tracking number 202501h3. EnhiA.01648.a.D11 PD00049 (VCID2443) at 12 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 290K