3SHU
Crystal structure of ZO-1 PDZ3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.030, 76.840, 39.110 |
| Unit cell angles | 90.00, 113.04, 90.00 |
Refinement procedure
| Resolution | 38.420 - 2.750 |
| R-factor | 0.21991 |
| Rwork | 0.215 |
| R-free | 0.26385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.840 | |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.121 | |
| Number of reflections | 4871 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% (w/v) PEG 4000, 10% (v/v) 2-Propanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
