3SHQ

Crystal Structure of UBLCP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	101.813, 101.813, 90.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.996 - 1.960
R-factor	0.1826
Rwork	0.181
R-free	0.20750
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.948
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.990
High resolution limit [Å]	1.960	5.320	1.960
Rmerge	0.079	0.050	0.474
Number of reflections	34990
<I/σ(I)>	9.8
Completeness [%]	100.0	99.9	100
Redundancy	9.5	8.6	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop	6.5	277	PEG 3350, Na-Formate, pH 6.5, sitting drop, temperature 277K
1	sitting drop	6.5	277	PEG 3350, Na-Formate, pH 6.5, sitting drop, temperature 277K