3SH8
Crystal structure of fluorophore-labeled beta-lactamase PenP in complex with cephaloridine
Replaces: 3KGOExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 291 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.330, 91.730, 66.110 |
| Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
| Resolution | 24.600 - 2.000 |
| R-factor | 0.22762 |
| Rwork | 0.225 |
| R-free | 0.26746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.201 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.060 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 46065 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
