3SGS
Amyloid-related segment of alphaB-crystallin residues 95-100
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 4.821, 19.500, 21.004 |
| Unit cell angles | 90.00, 94.23, 90.00 |
Refinement procedure
| Resolution | 14.270 - 1.703 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.830 |
| High resolution limit [Å] | 1.700 | 2.910 | 1.700 |
| Rmerge | 0.178 | 0.146 | 0.386 |
| Number of reflections | 438 | ||
| <I/σ(I)> | 24.2 | ||
| Completeness [%] | 97.6 | 96 | 100 |
| Redundancy | 4.9 | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.1M DL-MALIC ACID pH 7.0, vapor diffusion, hanging drop, temperature 298K |
