3SGO
Amyloid-related segment of alphaB-crystallin residues 90-100
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 69.284, 69.284, 69.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.970 - 2.557 |
R-factor | 0.2453 |
Rwork | 0.244 |
R-free | 0.26970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.022 |
RMSD bond angle | 1.718 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0061) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
Rmerge | 0.075 | 0.026 | 0.600 |
Number of reflections | 973 | ||
<I/σ(I)> | 15.5 | ||
Completeness [%] | 92.0 | 82.2 | 98 |
Redundancy | 7.9 | 6.9 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M BIS-TRIS pH 6.5, 45% MPD, 0.2M AMMONIUM ACETATE, vapor diffusion, hanging drop, temperature 298K |