3SG9
Crystal Structure of Aminoglycoside-2''-Phosphotransferase Type IVa Kanamycin A Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-03-16 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.903, 101.426, 73.399 |
| Unit cell angles | 90.00, 100.61, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.19829 |
| Rwork | 0.195 |
| R-free | 0.25052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.871 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.090 | 2.150 |
| Number of reflections | 32322 | |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 50 mM HEPES, 17% PEG4000, 10% glycerol, 5% isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
