3SG5
Crystal Structure of Dimeric GCaMP3-D380Y, QP(linker 1), LP(linker 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.567, 46.005, 67.365 |
| Unit cell angles | 90.00, 100.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.18216 |
| Rwork | 0.180 |
| R-free | 0.22552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek7 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.899 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 29184 | |
| <I/σ(I)> | 10.9 | 3.5 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris, pH 6.5, 2 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
