3SF5

Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-04-21
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	70.653, 70.732, 205.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.827 - 2.495
R-factor	0.1754
Rwork	0.173
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o1q
RMSD bond length	0.003
RMSD bond angle	0.692
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.050	2.650
High resolution limit [Å]	2.495	2.830	2.500
Number of reflections	36388
Completeness [%]	99.1	99.9	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	16% PEG 4000, 0.15M ammonium sulfate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K