3SE1
Frog M-ferritin with magnesium, R72D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 183.560, 183.560, 183.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.670 - 1.650 |
| R-factor | 0.17368 |
| Rwork | 0.172 |
| R-free | 0.20329 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.443 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.102 | 0.119 |
| Number of reflections | 32417 | |
| <I/σ(I)> | 22.6 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2.0 M MgCl2, 100 mM BICINE, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
