3SD4
Crystal structure of the first Tudor domain of human PHF20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.746, 60.761, 37.750 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 30.380 - 1.928 |
| R-factor | 0.2295 |
| Rwork | 0.229 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model generated using PHYRE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.877 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.380 | 1.960 |
| High resolution limit [Å] | 1.928 | 1.928 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 11693 | |
| <I/σ(I)> | 16.3 | 7.2 |
| Completeness [%] | 98.1 | 95.9 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 28% PEG 3000, 0.2 M ammonium acetate, 0.1 M cacodylic acid , pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
