3SCN
Crystal Structure of Rice BGlu1 E386G Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2008-08-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.382, 100.828, 127.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.840 - 2.200 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.350 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.5.0110) |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 51498 | |
<I/σ(I)> | 13.9 | 4.4 |
Completeness [%] | 97.5 | 92.6 |
Redundancy | 5.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 24% PEG MME 5000, 0.16M ammonium sulfate, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |