3SBU

Crystal structure of a ntf2-like protein (BF2862) from Bacteroides fragilis NCTC 9343 at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 61
Unit cell lengths	80.485, 80.485, 196.096
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.591 - 2.150
R-factor	0.1769
Rwork	0.176
R-free	0.19170
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.591	29.591	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge		0.027	0.818
Total number of observations		2241	16463
Number of reflections	38981
<I/σ(I)>	14.9	71.8	2
Completeness [%]	99.9	92.8	100
Redundancy	5.8	5.2	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	30.0% Glycerol, 5.6% polyethylene glycol 4000, 1.0M lithium chloride, 0.1M sodium citrate pH 5.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 293K
2