3SBS
Crystal structure of Aar2 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.772, 101.772, 119.749 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.937 - 2.100 |
R-factor | 0.1667 |
Rwork | 0.165 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.188 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.500 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 37260 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 277 | 0.1 M sodium acetate, pH 5.0, 2.0 M sodium formate, VAPOR DIFFUSION, temperature 277K |