3SBQ
Pseudomonas stutzeri nitrous oxide reductase, P65 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 70.373, 70.373, 400.104 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.18951 |
| Rwork | 0.188 |
| R-free | 0.21276 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fwx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 66.680 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.096 |
| Number of reflections | 114995 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 98.3 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 16 % PEG 6000, 0.2 M imidazole/malate buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
