3SBP

Pseudomonas stutzeri nitrous oxide reductase, P1 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-12-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	96.890, 106.700, 131.139
Unit cell angles	111.34, 107.33, 90.74

Refinement procedure

Resolution	46.020 - 2.100
R-factor	0.18007
Rwork	0.177
R-free	0.23554
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fwx
RMSD bond length	0.010
RMSD bond angle	1.330
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.100
High resolution limit [Å]	2.100
Rmerge	0.107
Number of reflections	242775
<I/σ(I)>	16.7
Completeness [%]	94.7
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	16 % PEG 6000, 0.2 M imidazole/malate buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K