3SBK
Russell's viper venom serine proteinase, RVV-V (PPACK-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 77.233, 77.233, 168.433 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.740 - 2.550 |
| R-factor | 0.2501 |
| Rwork | 0.246 |
| R-free | 0.32760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s9a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.895 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.065 | 0.267 |
| Number of reflections | 10311 | |
| <I/σ(I)> | 33.8 | 15.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 20.4 | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 9.6% PEG 3350, 0.8% tryptone, 40mM Na/HEPES , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
