3SBF

Crystal structure of the mutant P311A of enolase superfamily member from VIBRIONALES BACTERIUM complexed with Mg and D-Arabinonate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 42 21 2
Unit cell lengths	163.372, 163.372, 110.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.794 - 1.500
R-factor	0.1811
Rwork	0.180
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r25
RMSD bond length	0.006
RMSD bond angle	1.065
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.794
High resolution limit [Å]	1.500
Number of reflections	232178
Completeness [%]	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% PEG 3350, 0.1M Hepes, 0.2M Proline, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K