3SB9

Cu-mediated Dimer of T4 Lysozyme R76H/R80H by Synthetic Symmetrization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 41
Unit cell lengths	79.114, 79.114, 87.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.114 - 2.450
R-factor	0.1943
Rwork	0.192
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.074
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	2.540
High resolution limit [Å]	2.450	5.280	2.450
Rmerge	0.088	0.036	0.529
Number of reflections	19881
<I/σ(I)>	9.1
Completeness [%]	99.9	99.5	100
Redundancy	3.6	3.5	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	3.5M Sodium Formate, pH 7.0, vapor diffusion, hanging drop, temperature 290K