3SB2
Crystal Structure of the RNA chaperone Hfq from Herbaspirillum seropedicae SMR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.639, 65.323, 60.987 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 23.467 - 2.630 |
| R-factor | 0.2029 |
| Rwork | 0.199 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.541 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.780 | 2.700 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 13212 | |
| <I/σ(I)> | 29.21 | 6.52 |
| Completeness [%] | 97.2 | 94.9 |
| Redundancy | 6.02 | 5.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M PCB pH 7.0 and 25% (w/v) PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
