3SAW

MUTM Slanted complex 8 with R112A mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	45.295, 97.299, 102.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.300 - 2.350
R-factor	0.187
Rwork	0.185
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f5o
RMSD bond length	0.007
RMSD bond angle	1.111
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.066	0.417
Number of reflections	20503
<I/σ(I)>	11.9
Completeness [%]	98.2	99.6
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	273	PEG 8K, SODIUM CACODYLATE, GLYCEROL, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 273K