3SAF

Crystal structure of the human RRP6 catalytic domain with D313N mutation in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-12
Detector	ADSC QUANTUM 315
Spacegroup name	P 4
Unit cell lengths	138.900, 138.900, 58.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.740 - 2.500
Rwork	0.205
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hbj
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.067	0.036	0.421
Number of reflections	75251
<I/σ(I)>	15
Completeness [%]	99.3	99.6	99.2
Redundancy	3.8	4.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	6% PEG6000, 1.5 M sodium chloride, 0.4 mM yttrium(III) trichloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K