3S9G
Structure of human Hexim1 (delta stammer) coiled coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97943 |
| Spacegroup name | I 4 |
| Unit cell lengths | 70.770, 70.770, 90.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 2.100 |
| R-factor | 0.23904 |
| Rwork | 0.236 |
| R-free | 0.29580 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.031 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.386 |
| Number of reflections | 13117 | |
| <I/σ(I)> | 14.7 | 3.55 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M sodium cacodylate, 0.1 M calcium acetate, 4% PEG 8000, 5 mM magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
