3S97
PTPRZ CNTN1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 334.108, 45.957, 78.947 |
| Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
| Resolution | 33.561 - 2.297 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 3JXF and 1CS6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.297 | 4.950 | 2.297 |
| Rmerge | 0.153 | 0.088 | 0.493 |
| Number of reflections | 51002 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 96.4 | 99.6 | 81.3 |
| Redundancy | 6.9 | 7.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% (w/v) PEG 1,500, 50 mM Na-cacodylate pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
