3S93

Crystal structure of conserved motif in TDRD5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-18
Detector	RIGAKU SATURN
Spacegroup name	P 21 21 21
Unit cell lengths	42.940, 43.330, 126.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.480 - 2.280
R-factor	0.246
Rwork	0.245
R-free	0.27100
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.060
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.038	0.791
Number of reflections	10833
<I/σ(I)>	33.8	2.8
Completeness [%]	94.8	76.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			291	crystal used for final refinement: 1.5 M ammonium phosphate, 0.1 M bis-tris propane. 1:200 trypsin was also added., pH 8.5, vapor diffusion, temperature 291K
2			291	Selenomethionyl derivative: 1.5 M ammonium phosphat e, 0.1 M bis-tris propane. 1:200 trypsin was also added., pH 7, vapor diffusion, temperature 291K
3			291	crystal used for preliminary refinement: 25 w/v% PEG-3350, 0.2 M lithium sulfate, 0.1 M bis-tris propane. 1:200 trypsin was also added., pH 6.5, vapor diffusion, temperature 291K