3S8Z
Crystal structure of LRP6-E3E4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.600, 83.750, 166.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.25821 |
| Rwork | 0.257 |
| R-free | 0.28197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ijq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.125 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 28053 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM ADA pH 6.5, 100mM MgCl2, 12% PEG6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
